1N7D

Extracellular domain of the LDL receptor

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Collection date	2001-12-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.21363, 1.21423, 1.10696, 1.23985
Spacegroup name	P 31 2 1
Unit cell lengths	185.290, 185.290, 85.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.100 * - 3.700
Rwork	0.381
R-free	0.39200 *
Structure solution method	MAD
Starting model (for MR)	PDB ENTRIES: 1AJJ 1d2j 1i0u 1ijq 1fx5
RMSD bond length	0.030 *
RMSD bond angle	26.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700 *	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.090	0.449
Total number of observations	64801 *
Number of reflections	17303
<I/σ(I)>	9.8	2.7
Completeness [%]	95.6	64.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.3	298	acetate buffer pH 5.3, 1,2-hexanediol, 0.5 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	unknown *	5.3	298	acetate buffer pH 5.3, 1,2-hexanediol, 0.5 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	unknown *	5.3	298	acetate buffer pH 5.3, 1,2-hexanediol, 0.5 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium phosphate	25 (mM)	pH8.
2	1	1		150 (mM)
3	1	2	sodium acetate	50 (mM)	pH5.3
4	1	2	1,2-hexanediol	3 (%)
5	1	2		0.5 (mM)