1N1I

The structure of MSP-1(19) from Plasmodium knowlesi

Summary for 1N1I

• Entry DOI: 10.2210/pdb1n1i/pdb
• Related: 1B9W 1CEJ
• Descriptor: Merozoite surface protein-1, IMIDAZOLE, HISTIDINE, ... (4 entities in total)
• Functional Keywords: msp1, malaria, surface protein, surface antigen, glycoprotein, egf domain, cell adhesion
• Biological source: Plasmodium knowlesi strain H
• Total number of polymer chains: 4
• Total formula weight: 46096.90

Authors

Garman, S.C.,Simcoke, W.N.,Stowers, A.W.,Garboczi, D.N. (deposition date: 2002-10-17, release date: 2003-02-25, Last modification date: 2024-11-20)

Primary citation

Garman, S.C.,Simcoke, W.N.,Stowers, A.W.,Garboczi, D.N.
Structure of the C-terminal domains of merozoite surface protein-1 from Plasmodium knowlesi reveals a novel histidine binding site
J.Biol.Chem., 278:7264-7269, 2003

PubMed Abstract: The protozoan parasite Plasmodium causes malaria, with hundreds of millions of cases recorded annually. Protection against malaria infection can be conferred by antibodies against merozoite surface protein (MSP)-1, making it an attractive vaccine candidate. Here we present the structure of the C-terminal domains of MSP-1 (known as MSP-1(19)) from Plasmodium knowlesi. The structure reveals two tightly packed epidermal growth factor-like domains oriented head to tail. In domain 1, the molecule displays a histidine binding site formed primarily by a highly conserved tryptophan. The protein carries a pronounced overall negative charge primarily due to the large number of acidic groups in domain 2. To map protein binding surfaces on MSP-1(19), we have analyzed the crystal contacts in five different crystal environments, revealing that domain 1 is highly preferred in protein-protein interactions. A comparison of MSP-1(19) structures from P. knowlesi, P. cynomolgi, and P. falciparum shows that, although the overall protein folds are similar, the molecules show significant differences in charge distribution. We propose the histidine binding site in domain 1 as a target for inhibitors of protein binding to MSP-1, which might prevent invasion of the merozoite into red blood cells.

PubMed: 12493733
DOI: 10.1074/jbc.M210716200

Experimental method

X-RAY DIFFRACTION (2.4 Å)