1N1I

The structure of MSP-1(19) from Plasmodium knowlesi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-02-23
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	33.880, 106.460, 62.690
Unit cell angles	90.00, 102.05, 90.00

Refinement procedure

Resolution	50.000 * - 2.400
R-factor	0.234
Rwork	0.234
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9w
RMSD bond length	0.008
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.082	0.315
Total number of observations	56495 *
Number of reflections	16570 *	1565 *
<I/σ(I)>	14.9	3.4
Completeness [%]	97.7 *	93.7
Redundancy	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	293	PEG 6000, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	30 (%)
3	1	reservoir	HEPES	100 (mM)	pH7.0