1MX3

Crystal structure of CtBP dehydrogenase core holo form

1MX3 の概要

• エントリーDOI: 10.2210/pdb1mx3/pdb
• 分子名称: C-terminal binding protein 1, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ACETIC ACID, ... (4 entities in total)
• 機能のキーワード: nuclear protein, phosphorylation, transcriptional corepressor, transcription repressor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q13363
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38862.95

構造登録者

Kumar, V.,Carlson, J.E.,Ohgi, K.E.,Edwards, T.E.,Rose, D.W.,Escalante, C.R.,Aggarwal, A.K. (登録日: 2002-10-01, 公開日: 2002-12-18, 最終更新日: 2024-02-14)

主引用文献

Kumar, V.,Carlson, J.E.,Ohgi, K.E.,Edwards, T.E.,Rose, D.W.,Escalante, C.R.,Aggarwal, A.K.
Transcription Corepressor CtBP Is an NAD+-Regulated Dehydrogenase
Mol.Cell, 10:857-869, 2002

PubMed Abstract: Transcriptional repression is based on the selective actions of recruited corepressor complexes, including those with enzymatic activities. One well-characterized developmentally important corepressor is the C-terminal binding protein (CtBP). Although intriguingly related in sequence to D2 hydroxyacid dehydrogenases, the mechanism by which CtBP functions remains unclear. We report here biochemical and crystallographic studies which reveal that CtBP is a functional dehydrogenase. In addition, both a cofactor-dependent conformational change, with NAD(+) and NADH being equivalently effective, and the active site residues are linked to the binding of the PXDLS consensus recognition motif on repressors, such as E1A and RIP140. Together, our data suggest that CtBP is an NAD(+)-regulated component of critical complexes for specific repression events in cells.

PubMed: 12419229
DOI: 10.1016/S1097-2765(02)00650-0

実験手法

X-RAY DIFFRACTION (1.95 Å)