1MX3

Crystal structure of CtBP dehydrogenase core holo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Wavelength(s)	1.140
Spacegroup name	P 64 2 2
Unit cell lengths	89.100, 89.100, 164.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.950
Rwork	0.211
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.530 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Rmerge	0.041	0.314 *
Total number of observations	173537 *
Number of reflections	52854
Completeness [%]	99.0	94.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		sodium formate, magnesium acetate, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP
1	unknown *	7		sodium formate, magnesium acetate, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP
1	unknown *	7		sodium formate, magnesium acetate, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium formate	140 (mM)
2	1	1	magnesium acetate	70 (mM)
3	1	1	HEPES	100 (mM)	pH7.0