1MW8

Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'

1MW8 の概要

• エントリーDOI: 10.2210/pdb1mw8/pdb
• 分子名称: 5'-D(*AP*CP*TP*TP*CP*GP*GP*GP*AP*TP*G)-3', DNA Topoisomerase I, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: dna topoisomerase, decatenase enzyme, toprim domain, isomerase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71122.56

構造登録者

Perry, K.,Mondragon, A. (登録日: 2002-09-27, 公開日: 2003-10-14, 最終更新日: 2024-04-03)

主引用文献

Perry, K.,Mondragon, A.
Structure of a Complex between E. coli DNA Topoisomerase I and Single-Stranded DNA.
Structure, 11:1349-1358, 2003

PubMed Abstract: In order to gain insights into the mechaism of ssDNA binding and recognition by Escherichia coli DNA topoisomerase I, the structure of the 67 kDa N-terminal fragment of topoisomerase I was solved in complex with ssDNA. The structure reveals a new conformational stage in the multistep catalytic cycle of type IA topoisomerases. In the structure, the ssDNA binding groove leading to the active site is occupied, but the active site is not fully formed. Large conformational changes are not seen; instead, a single helix parallel to the ssDNA binding groove shifts to clamp the ssDNA. The structure helps clarify the temporal sequence of conformational events, starting from an initial empty enzyme and proceeding to a ssDNA-occupied and catalytically competent active site.

PubMed: 14604525
DOI: 10.1016/j.str.2003.09.013

実験手法

X-RAY DIFFRACTION (1.9 Å)