1MTL

Non-productive MUG-DNA complex

Summary for 1MTL

• Entry DOI: 10.2210/pdb1mtl/pdb
• Related: 1MUG
• Descriptor: 5'-D(*CP*GP*CP*GP*AP*GP*(AAB)P*TP*CP*GP*CP*G)-3', G/U mismatch-specific DNA glycosylase (3 entities in total)
• Functional Keywords: glycosylase, cis-platin, inter-strand, non-productive, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli
• Cellular location: Cytoplasm : P0A9H1
• Total number of polymer chains: 4
• Total formula weight: 44535.29

Authors

Barrett, T.E.,Savva, R.,Barlow, T.,Brown, T.,Jiricny, J.,Pearl, L.H. (deposition date: 2002-09-21, release date: 2002-09-27, Last modification date: 2024-04-03)

Primary citation

Barrett, T.E.,Savva, R.,Barlow, T.,Brown, T.,Jiricny, J.,Pearl, L.H.
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct.
Nat.Struct.Biol., 5:697-701, 1998

PubMed Abstract: Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.

PubMed: 9699633
DOI: 10.1038/1394

Experimental method

X-RAY DIFFRACTION (2.8 Å)