1MTL
Non-productive MUG-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.620, 84.860, 97.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.800 |
| R-factor | 0.275 |
| Rwork | 0.206 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native MUG structure |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.062 | 0.186 |
| Number of reflections | 13290 * | |
| <I/σ(I)> | 6.5 | 4 |
| Completeness [%] | 94.0 | 96.5 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.7 * | 289 | PEG 4000, Ammonium sulphate, Sodium acetate, pH 4.6, Microbatch, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Ammonium sulphate | ||
| 3 | 1 | 1 | Sodium acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | PEG4000 | 8 (%(w/v)) | ||
| 2 | 1 | ammonium sulfate | 100 (mM) | ||
| 3 | 1 | sodium acetate | 50 (mM) | pH4.7 | |
| 4 | 1 | 2 | protein | 13 (mg/ml) |
