1ML3

Evidences for a flip-flop catalytic mechanism of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase, from its crystal structure in complex with reacted irreversible inhibitor 2-(2-phosphono-ethyl)-acrylic acid 4-nitro-phenyl ester

Summary for 1ML3

Entry DOI	10.2210/pdb1ml3/pdb
Related	1A7K 1GYP
Descriptor	Glyceraldehyde 3-phosphate dehydrogenase, glycosomal, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, (3-FORMYL-BUT-3-ENYL)-PHOSPHONIC ACID, ... (4 entities in total)
Functional Keywords	protein covalent-inhibitor complex, oxidoreductase
Biological source	Trypanosoma cruzi
Cellular location	Glycosome: P22513
Total number of polymer chains	4
Total formula weight	158932.72
Authors	Castilho, M.S.,Pavao, F.,Oliva, G. (deposition date: 2002-08-29, release date: 2003-07-08, Last modification date: 2024-04-03)
Primary citation	Castilho, M.S.,Pavao, F.,Oliva, G.,Ladame, S.,Willson, M.,Perie, J. Evidence for the Two Phosphate Binding Sites of an Analogue of the Thioacyl Intermediate for the Trypanosoma cruzi Glyceraldehyde-3-phosphate Dehydrogenase-Catalyzed Reaction, from Its Crystal Structure. Biochemistry, 42:7143-7151, 2003 Cited by PubMed: 12795610 DOI: 10.1021/bi0206107 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation