1MJ1

FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME

Summary for 1MJ1

• Entry DOI: 10.2210/pdb1mj1/pdb
• EMDB information: 1004
• Descriptor: Phe-tRNA, sarcin-ricin loop of 23SrRNA, helix 69 of 23S rRNA, ... (7 entities in total)
• Functional Keywords: 70s ribosome, low resolution model ternary complex, ef-tu, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 8
• Total formula weight: 162273.77

Authors

Stark, H.,Rodnina, M.V.,Wieden, H.-J.,Zemlin, F.,Wintermeyer, W.,Vanheel, M. (deposition date: 2002-08-26, release date: 2002-11-01, Last modification date: 2024-02-14)

Primary citation

Stark, H.,Rodnina, M.V.,Wieden, H.-J.,Zemlin, F.,Wintermeyer, W.,van Heel, M.
Ribosome Interactions of Aminoacyl-tRNA and Elongation Factor TU in the Codon Recognition Complex
Nat.Struct.Biol., 9:849-854, 2002

PubMed Abstract: The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.

PubMed: 12379845

Experimental method

ELECTRON MICROSCOPY (13 Å)