1MHP

Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody

1MHP の概要

• エントリーDOI: 10.2210/pdb1mhp/pdb
• 分子名称: integrin alpha 1, (RESIDUES 169-360), Fab fragment, heavy chain, FAB FRAGMENT, light chain, ... (4 entities in total)
• 機能のキーワード: integrin, cell adhesion, receptor, antibody, immune system
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P18614
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 136056.04

構造登録者

Karpusas, M.,Taylor, F.,Ferrant, J.,Weinreb, P.,Garber, E. (登録日: 2002-08-20, 公開日: 2003-04-15, 最終更新日: 2024-11-06)

主引用文献

Karpusas, M.,Ferrant, J.,Weinreb, P.,Carmillo, A.,Taylor, F.,Garber, E.
Crystal Structure of the alpha 1 beta 1 Integrin I Domain in Complex with an Antibody Fab Fragment
J.Mol.Biol., 327:1031-1041, 2003

PubMed Abstract: The alpha1beta1 (VLA-1) integrin is a cell-surface receptor for collagen and laminin and has been implicated in biological pathways involved in several pathological processes. These processes may be inhibited by the monoclonal antibody AQC2, which binds with high affinity to human alpha1beta1 integrin. To understand the structural basis of the inhibition we determined the crystal structure of the complex of a chimeric rat/human I domain of the alpha1beta1 integrin and the Fab fragment of humanized AQC2 antibody. The structure of the complex shows that the antibody blocks the collagen binding site of the I domain. An aspartate residue, from the CDR3 loop of the antibody heavy chain, coordinates the MIDAS metal ion in a manner similar to that of a glutamate residue from collagen. Substitution of the aspartate residue by alanine or arginine results in significant reduction of antibody binding affinity. Interestingly, although the mode of metal ion coordination resembles that of the open conformation, the I domain maintains an overall closed conformation previously observed only for unliganded I domains.

PubMed: 12662928
DOI: 10.1016/S0022-2836(03)00203-1

実験手法

X-RAY DIFFRACTION (2.8 Å)