1M3K

biosynthetic thiolase, inactive C89A mutant

1M3K の概要

• エントリーDOI: 10.2210/pdb1m3k/pdb
• 関連するPDBエントリー: 1DLU 1DLV 1DM3 1M1O 1M1T 1M3Z 1M4S 1M4T 1QFL
• 分子名称: Acetyl-CoA acetyltransferase, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: thiolase fold, transferase
• 由来する生物種: Zoogloea ramigera
• 細胞内の位置: Cytoplasm: P07097
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 162605.02

構造登録者

Kursula, P.,Ojala, J.,Lambeir, A.-M.,Wierenga, R.K. (登録日: 2002-06-28, 公開日: 2002-11-29, 最終更新日: 2024-02-14)

主引用文献

Kursula, P.,Ojala, J.,Lambeir, A.-M.,Wierenga, R.K.
The catalytic cycle of biosynthetic thiolase: A conformational journey of an acetyl group through four binding modes and two oxyanion holes
Biochemistry, 41:15543-15556, 2002

PubMed Abstract: Biosynthetic thiolase catalyzes the formation of acetoacetyl-CoA from two molecules of acetyl-CoA. This is a key step in the synthesis of many biological compounds, including steroid hormones and ketone bodies. The thiolase reaction involves two chemically distinct steps; during acyl transfer, an acetyl group is transferred from acetyl-CoA to Cys89, and in the Claisen condensation step, this acetyl group is further transferred to a second molecule of acetyl-CoA, generating acetoacetyl-CoA. Here, new crystallographic data for Zoogloea ramigera biosynthetic thiolase are presented, covering all intermediates of the thiolase catalytic cycle. The high-resolution structures indicate that the acetyl group goes through four conformations while being transferred from acetyl-CoA via the acetylated enzyme to acetoacetyl-CoA. This transfer is catalyzed in a rigid cavity lined by mostly hydrophobic side chains, in addition to the catalytic residues Cys89, His348, and Cys378. The structures highlight the importance of an oxyanion hole formed by a water molecule and His348 in stabilizing the negative charge on the thioester oxygen atom of acetyl-CoA at two different steps of the reaction cycle. Another oxyanion hole, composed of the main chain nitrogen atoms of Cys89 and Gly380, complements a negative charge of the thioester oxygen anion of the acetylated intermediate, stabilizing the tetrahedral transition state of the Claisen condensation step. The reactivity of the active site may be modulated by hydrogen bonding networks extending from the active site toward the back of the molecule.

PubMed: 12501183
DOI: 10.1021/bi0266232

実験手法

X-RAY DIFFRACTION (1.7 Å)