1M3K

biosynthetic thiolase, inactive C89A mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
A	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
A	0044281	biological_process	small molecule metabolic process
B	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
B	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
B	0044281	biological_process	small molecule metabolic process
C	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
C	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
C	0044281	biological_process	small molecule metabolic process
D	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
D	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0042619	biological_process	poly-hydroxybutyrate biosynthetic process
D	0044281	biological_process	small molecule metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 9720

Chain	Residue
A	SER260
A	ARG266

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site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 9722

Chain	Residue
A	LYS298
A	ARG302
A	HOH442

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site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 5393

Chain	Residue
A	HOH511
A	HOH516
A	HIS156
A	SER247
A	GLY248
A	HOH502

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 9719

Chain	Residue
B	LYS298
B	ARG302
B	HOH9893

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 9721

Chain	Residue
B	SER260
B	ARG266
B	HOH9865

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 6393

Chain	Residue
B	HIS156
B	SER247
B	LEU249
B	PHE319
B	HOH504

---

Functional Information from PROSITE/UniProt

site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. GLATLCIGgGmGvA

Chain	Residue	Details
A	GLY373-ALA386

---

site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NvnGGaIAiGHPiGaSG

Chain	Residue	Details
A	ASN338-GLY354

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Acyl-thioester intermediate

Chain	Residue	Details
A	ALA89
B	ALA89
C	ALA89
D	ALA89

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	HIS348
A	CYS378
B	HIS348
B	CYS378
C	HIS348
C	CYS378
D	HIS348
D	CYS378

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1qfl

Chain	Residue	Details
A	HIS348
A	CYS378

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site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1qfl

Chain	Residue	Details
B	HIS348
B	CYS378

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1qfl

Chain	Residue	Details
C	HIS348
C	CYS378

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1qfl

Chain	Residue	Details
D	HIS348
D	CYS378

---