1M1F
Kid toxin protein from E.coli plasmid R1
Summary for 1M1F
Entry DOI | 10.2210/pdb1m1f/pdb |
Related | 4VUB |
Descriptor | Kid toxin protein, PHOSPHATE ION (3 entities in total) |
Functional Keywords | toxin-antitoxin, plasmid maintenance, post segregational killing, dna replication, mutational analysis, ccdb, toxin |
Biological source | Escherichia coli |
Total number of polymer chains | 2 |
Total formula weight | 23886.50 |
Authors | Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B. (deposition date: 2002-06-19, release date: 2002-11-20, Last modification date: 2024-02-14) |
Primary citation | Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B. Structural and Functional Analysis of the Kid toxin protein from E.coli plasmid R1 Structure, 10:1425-1433, 2002 Cited by PubMed Abstract: We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action. PubMed: 12377128DOI: 10.1016/S0969-2126(02)00856-0 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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