1M1F

Kid toxin protein from E.coli plasmid R1

Experimental procedure

Experimental method	3 WAVELENGTH MA
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97965, 0.97979, 0.96863
Spacegroup name	P 1 21 1
Unit cell lengths	33.264, 45.457, 65.021
Unit cell angles	90.00, 96.12, 90.00

Refinement procedure

Resolution	12.000 - 1.250 *
R-factor	0.16126
Rwork	0.160
R-free	0.18200 *
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.336
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000 *	1.430
High resolution limit [Å]	1.250 *	1.390
Rmerge	0.042	0.110
Number of reflections	43111
<I/σ(I)>	3	3
Completeness [%]	93.0	87.8
Redundancy	5.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	290	Hargreaves, D., (2002) Acta Crystallogr., D58, 355. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.4-1.5 (M)
2	1	drop	potassium phosphate	100 (mM)	pH5.8
3	1	drop	protein	2-2.5 (mg/ml)