1M1A

LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA

1M1A の概要

• エントリーDOI: 10.2210/pdb1m1a/pdb
• 関連するPDBエントリー: 1AOI 1M18 1M19
• 分子名称: Palindromic 146 Base Pair DNA Fragment, BETA-ALANINE, 3-AMINO-(DIMETHYLPROPYLAMINE), ... (12 entities in total)
• 機能のキーワード: nucleosome, chromatin, histone, pyrrole-imidazole polyamide, dna regognition, chromatin remodeling, structural protein-dna complex, structural protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: 1M1A P02302 A0A8J1LTD2 P06897
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 200863.76

構造登録者

Suto, R.K.,Edayathumangalam, R.S.,White, C.L.,Melander, C.,Gottesfeld, J.M.,Dervan, P.B.,Luger, K. (登録日: 2002-06-18, 公開日: 2003-02-18, 最終更新日: 2024-02-14)

主引用文献

Suto, R.K.,Edayathumangalam, R.S.,White, C.L.,Melander, C.,Gottesfeld, J.M.,Dervan, P.B.,Luger, K.
Crystal Structures of Nucleosome Core Particles in Complex with Minor Groove DNA-binding Ligands
J.MOL.BIOL., 326:371-380, 2003

PubMed Abstract: We determined the crystal structures of three nucleosome core particles in complex with site-specific DNA-binding ligands, the pyrrole-imidazole polyamides. While the structure of the histone octamer and its interaction with the DNA remain unaffected by ligand binding, nucleosomal DNA undergoes significant structural changes at the ligand-binding sites and in adjacent regions to accommodate the ligands. Our findings suggest that twist diffusion occurs over long distances through tightly bound nucleosomal DNA. This may be relevant to the mechanism of ATP-dependent and spontaneous nucleosome translocation, and to the effect of bound factors on nucleosome dynamics.

PubMed: 12559907
DOI: 10.1016/S0022-2836(02)01407-9

実験手法

X-RAY DIFFRACTION (2.65 Å)