1M1A
LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.719, 109.196, 177.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 2.650 |
Rwork | 0.223 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.240 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.064 | 0.286 |
Number of reflections | 61273 * | |
<I/σ(I)> | 18.2 | 2.1 |
Completeness [%] | 99.4 * | 96.9 |
Redundancy | 19.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 40-45 (mM) | ||
2 | 1 | reservoir | 35-38.8 (mM) | ||
3 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
4 | 1 | drop | protein | 4 (mg/ml) |