1M19

LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA

Summary for 1M19

• Entry DOI: 10.2210/pdb1m19/pdb
• Related: 1AOI 1M18 1M1A
• Descriptor: Palindromic 146 Base Pair DNA Fragment, BETA-ALANINE, 3-AMINO-(DIMETHYLPROPYLAMINE), ... (12 entities in total)
• Functional Keywords: nucleosome, chromatin, histone, pyrrole-imidazole polyamide, dna regognition, chromatin remodeling, structural protein-dna complex, structural protein/dna
• Biological source: Xenopus laevis (African clawed frog); More
• Cellular location: Nucleus: 1M19 P02302 A0A8J1LTD2 P06897
• Total number of polymer chains: 10
• Total formula weight: 206583.70

Authors

Suto, R.K.,Edayathumangalam, R.S.,White, C.L.,Melander, C.,Gottesfeld, J.M.,Dervan, P.B.,Luger, K. (deposition date: 2002-06-18, release date: 2003-02-18, Last modification date: 2023-11-15)

Primary citation

Suto, R.K.,Edayathumangalam, R.S.,White, C.L.,Melander, C.,Gottesfeld, J.M.,Dervan, P.B.,Luger, K.
Crystal Structures of Nucleosome Core Particles in Complex with Minor Groove DNA-binding Ligands
J.Mol.Biol., 326:371-380, 2003

PubMed Abstract: We determined the crystal structures of three nucleosome core particles in complex with site-specific DNA-binding ligands, the pyrrole-imidazole polyamides. While the structure of the histone octamer and its interaction with the DNA remain unaffected by ligand binding, nucleosomal DNA undergoes significant structural changes at the ligand-binding sites and in adjacent regions to accommodate the ligands. Our findings suggest that twist diffusion occurs over long distances through tightly bound nucleosomal DNA. This may be relevant to the mechanism of ATP-dependent and spontaneous nucleosome translocation, and to the effect of bound factors on nucleosome dynamics.

PubMed: 12559907
DOI: 10.1016/S0022-2836(02)01407-9

Experimental method

X-RAY DIFFRACTION (2.3 Å)