1M04

Mutant Streptomyces plicatus beta-hexosaminidase (D313N) in complex with product (GlcNAc)

1M04 の概要

• エントリーDOI: 10.2210/pdb1m04/pdb
• 関連するPDBエントリー: 1M01 1M03 1hp5 1jak
• 分子名称: Beta-N-acetylhexosaminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: substrate assisted catalysis, hexosaminidase, family 20 glycosidase, hydrolase
• 由来する生物種: Streptomyces plicatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56641.52

構造登録者

Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.G.,Withers, S.G. (登録日: 2002-06-11, 公開日: 2002-12-11, 最終更新日: 2021-10-27)

• 主引用文献: Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.,Withers, S.G.; Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.; J.Biol.Chem., 277:40055-40065, 2002; PubMed: 12171933; DOI: 10.1074/jbc.M206481200

実験手法

X-RAY DIFFRACTION (1.95 Å)