1LUJ

Crystal Structure of the Beta-catenin/ICAT Complex

1LUJ の概要

• エントリーDOI: 10.2210/pdb1luj/pdb
• 関連するPDBエントリー: 1g3j 1i7w 1i7x 1jdh 1jpw
• 分子名称: Catenin beta-1, Beta-catenin-interacting protein 1 (2 entities in total)
• 機能のキーワード: beta-catenin, icat, wnt pathway, inhibitor, structural protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P35222 Q9JJN6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64495.70

構造登録者

Graham, T.A.,Clements, W.K.,Kimelman, D.,Xu, W. (登録日: 2002-05-22, 公開日: 2002-10-16, 最終更新日: 2024-02-14)

主引用文献

Graham, T.A.,Clements, W.K.,Kimelman, D.,Xu, W.
The crystal structure of the beta-catenin/ICAT complex reveals the inhibitory mechanism of ICAT.
Mol.Cell, 10:563-571, 2002

PubMed Abstract: Beta-catenin is a multifunctional protein involved in both cell adhesion and transcriptional activation. Transcription mediated by the beta-catenin/Tcf complex is involved in embryological development and is upregulated in various cancers. We have determined the crystal structure at 2.5 A resolution of a complex between beta-catenin and ICAT, a protein that prevents the interaction between beta-catenin and Tcf/Lef family transcription factors. ICAT contains a 3-helix bundle that binds armadillo repeats 10-12 and a C-terminal tail that, similar to Tcf and E-cadherin, binds in the groove formed by armadillo repeats 5-9 of beta-catenin. We show that ICAT selectively inhibits beta-catenin/Tcf binding in vivo, without disrupting beta-catenin/cadherin interactions. Thus, it should be possible to design cancer therapeutics that inhibit beta-catenin-mediated transcriptional activation without interfering with cell adhesion.

PubMed: 12408824
DOI: 10.1016/S1097-2765(02)00637-8

実験手法

X-RAY DIFFRACTION (2.5 Å)