1LUJ
Crystal Structure of the Beta-catenin/ICAT Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2002-02-16 |
Detector | SBC-2 |
Wavelength(s) | 0.9196 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.174, 98.504, 86.486 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.740 - 2.510 * |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 18.800 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.510 * | 2.500 |
Rmerge | 0.119 * | 0.650 * |
Total number of observations | 163681 * | |
Number of reflections | 23804 | |
<I/σ(I)> | 15.6 | 2.2 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG800, magnesium chloride, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 4 (%(w/v)) | |
3 | 1 | reservoir | 20 (mM) | ||
4 | 1 | reservoir | Tris | 50 (mM) | pH8.0 |
5 | 1 | reservoir | dithiothreitol | 5 (mM) |