1LTJ
Crystal Structure of Recombinant Human Fibrinogen Fragment D with the Peptide Ligands Gly-Pro-Arg-Pro-Amide and Gly-His-Arg-Pro-Amide
Summary for 1LTJ
Entry DOI | 10.2210/pdb1ltj/pdb |
Related | 1FZA 1FZB 1FZC 1FZE 1FZF 1FZG 1LT9 |
Descriptor | Fibrinogen alpha/alpha-E chain, Fibrinogen Beta chain, Fibrinogen Gamma chain, ... (8 entities in total) |
Functional Keywords | blood coagulation, fibrinogen, fibrinogen fragment d, recombinant fibrinogen fragment d, recombinant fibrinogen, recombinant fibrinogen fragment d with two peptide ligands, blood clotting |
Biological source | Homo sapiens (human) More |
Total number of polymer chains | 10 |
Total formula weight | 160899.89 |
Authors | Kostelansky, M.S.,Betts, L.,Gorkun, O.V.,Lord, S.T. (deposition date: 2002-05-20, release date: 2002-11-06, Last modification date: 2024-10-09) |
Primary citation | Kostelansky, M.S.,Betts, L.,Gorkun, O.V.,Lord, S.T. 2.8 A Crystal Structures of Recombinant Fibrinogen Fragment D with and without Two Peptide Ligands: GHRP Binding to the "b" Site Disrupts Its Nearby Calcium-binding Site. Biochemistry, 41:12124-12132, 2002 Cited by PubMed Abstract: We report two crystal structures, each at a resolution of 2.8 A, of recombinant human fibrinogen fragment D (rfD) in the absence and presence of peptide ligands. The bound ligands, Gly-Pro-Arg-Pro-amide and Gly-His-Arg-Pro-amide, mimic the interactions of the thrombin exposed polymerization sites, "A" and "B", respectively. This report is the first to describe the structure of fragment D in the presence of both peptide ligands. The structures reveal that recombinant fibrinogen is nearly identical to the plasma protein but with minor changes, like the addition of a proximal fucose to the carbohydrate linked to residue betaGln364, and slightly different relative positions of the beta- and gamma-modules. Of major interest in our structures is that a previously identified calcium site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound. The peptide-dependent loss of this calcium site may have significant biological implications that are further discussed. These structures provide a foundation for the detailed structural analysis of variant recombinant fibrinogens that were used to identify critical functional residues within fragment D. PubMed: 12356313DOI: 10.1021/bi0261894 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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