1FZB

CRYSTAL STRUCTURE OF CROSSLINKED FRAGMENT D

Summary for 1FZB

• Entry DOI: 10.2210/pdb1fzb/pdb
• Descriptor: FIBRINOGEN, PEPTIDE LIGAND GPRG, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: blood coagulation, plasma, platelet, fibrinogen, fibrin
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 169696.02

Authors

Spraggon, G.,Everse, S.J.,Doolittle, R.F. (deposition date: 1997-08-05, release date: 1997-12-03, Last modification date: 2024-10-30)

Primary citation

Spraggon, G.,Everse, S.J.,Doolittle, R.F.
Crystal structures of fragment D from human fibrinogen and its crosslinked counterpart from fibrin.
Nature, 389:455-462, 1997

PubMed Abstract: In blood coagulation, units of the protein fibrinogen pack together to form a fibrin clot, but a crystal structure for fibrinogen is needed to understand how this is achieved. The structure of a core fragment (fragment D) from human fibrinogen has now been determined to 2.9 A resolution. The 86K three-chained structure consists of a coiled-coil region and two homologous globular entitles oriented at approximately 130 degrees to each other. Additionally, the covalently bound dimer of fragment D, known as 'double-D', was isolated from human fibrin, crystallized in the presence of a Gly-Pro-Arg-Pro-amide peptide ligand, which simulates the donor polymerization site, and its structure solved by molecular replacement with the model of fragment D.

PubMed: 9333233
DOI: 10.1038/38947

Experimental method

X-RAY DIFFRACTION (2.9 Å)