1LNL
Structure of deoxygenated hemocyanin from Rapana thomasiana
Summary for 1LNL
| Entry DOI | 10.2210/pdb1lnl/pdb |
| Descriptor | hemocyanin, 2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION (3 entities in total) |
| Functional Keywords | hemocyanin, deoxygenated form, oxygen, metal binding site, cooperativity, oxygen storage-transport complex, oxygen storage/transport |
| Biological source | Rapana thomasiana |
| Total number of polymer chains | 3 |
| Total formula weight | 141607.95 |
| Authors | Perbandt, M.,Guthoehrlein, E.W.,Rypniewski, W.,Idakieva, K.,Stoeva, S.,Voelter, W.,Genov, N.,Betzel, C. (deposition date: 2002-05-03, release date: 2003-06-03, Last modification date: 2024-10-16) |
| Primary citation | Perbandt, M.,Guthoehrlein, E.W.,Rypniewski, W.,Idakieva, K.,Stoeva, S.,Voelter, W.,Genov, N.,Betzel, C. The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity Biochemistry, 42:6341-6346, 2003 Cited by PubMed Abstract: Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form. PubMed: 12767214DOI: 10.1021/bi020672x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.3 Å) |
Structure validation
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