1LMK

THE STRUCTURE OF A BIVALENT DIABODY

Summary for 1LMK

Entry DOI	10.2210/pdb1lmk/pdb
Descriptor	ANTI-PHOSPHATIDYLINOSITOL SPECIFIC PHOSPHOLIPASE C DIABODY (2 entities in total)
Functional Keywords	immunoglobulin, diabody, single-chain fv, scfv
Biological source	Mus musculus (house mouse)
Total number of polymer chains	4
Total formula weight	104312.48
Authors	Williams, R.L. (deposition date: 1994-08-29, release date: 1995-03-31, Last modification date: 2024-10-30)
Primary citation	Perisic, O.,Webb, P.A.,Holliger, P.,Winter, G.,Williams, R.L. Crystal structure of a diabody, a bivalent antibody fragment. Structure, 2:1217-1226, 1994 Cited by PubMed Abstract: Diabodies are dimeric antibody fragments. In each polypeptide, a heavy-chain variable domain (VH) is linked to a light-chain variable domain (VL) but unlike single-chain Fv fragments, each antigen-binding site is formed by pairing of one VH and one VL domain from the two different polypeptides. Diabodies thus have two antigen-binding sites, and can be bispecific. Direct structural evidence is lacking for the connections and dimeric interactions between the two polypeptides of the diabody. PubMed: 7704531 DOI: 10.1016/S0969-2126(94)00123-5 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation