1LMK
THE STRUCTURE OF A BIVALENT DIABODY
Experimental procedure
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.140, 80.710, 88.010 |
Unit cell angles | 90.00, 99.81, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.600 |
R-factor | 0.2 |
Rwork | 0.200 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.400 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 * |
Rmerge | 0.125 * |
Total number of observations | 124443 * |
Number of reflections | 31023 |
Completeness [%] | 98.4 |
Redundancy | 4. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.8 * | 17 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | ||
2 | 1 | drop | glycerol | 20 (%(v/v)) | precipitant |
3 | 1 | drop | PEG3000 | 15 (%(v/v)) | precipitant |
4 | 1 | drop | Tris-HCl | 0.1 (M) | precipitant |
5 | 1 | drop | sodium acetate | 0.2 (M) | precipitant |
6 | 1 | reservoir | precipitant |