1LM5

Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure

Summary for 1LM5

• Entry DOI: 10.2210/pdb1lm5/pdb
• Descriptor: subdomain of Desmoplakin Carboxy-Terminal domain (DPCT) (2 entities in total)
• Functional Keywords: plakin repeat, structural protein
• Biological source: Homo sapiens (human)
• Cellular location: Cell junction, desmosome: P15924
• Total number of polymer chains: 2
• Total formula weight: 46599.05

Authors

Choi, H.J.,Park-Snyder, S.,Pascoe, L.T.,Green, K.J.,Weis, W.I. (deposition date: 2002-04-30, release date: 2002-07-31, Last modification date: 2024-02-14)

Primary citation

Choi, H.J.,Park-Snyder, S.,Pascoe, L.T.,Green, K.J.,Weis, W.I.
Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure.
Nat.Struct.Biol., 9:612-620, 2002

PubMed Abstract: Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site.

PubMed: 12101406

Experimental method

X-RAY DIFFRACTION (1.8 Å)