1LM5
Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.320, 95.379, 54.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 * - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.031 * | 0.300 * |
| Total number of observations | 292583 * | |
| Number of reflections | 32732 * | |
| Completeness [%] | 96.1 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 20 * | PEG 4000, magnesium chloride, acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.2 |
| 3 | 1 | reservoir | PEG4000 | 31 (%(w/v)) | |
| 4 | 1 | reservoir | 150 (mM) |
