1LKI

THE CRYSTAL STRUCTURE AND BIOLOGICAL FUNCTION OF LEUKEMIA INHIBITORY FACTOR: IMPLICATIONS FOR RECEPTOR BINDING

1LKI の概要

• エントリーDOI: 10.2210/pdb1lki/pdb
• 分子名称: LEUKEMIA INHIBITORY FACTOR (2 entities in total)
• 機能のキーワード: cytokine, four helix bundle
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Secreted: P09056
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19891.01

構造登録者

Robinson, R.C.,Grey, L.M.,Staunton, D.,Stuart, D.I.,Heath, J.K.,Jones, E.Y. (登録日: 1994-12-12, 公開日: 1995-03-31, 最終更新日: 2024-06-05)

主引用文献

Robinson, R.C.,Grey, L.M.,Staunton, D.,Vankelecom, H.,Vernallis, A.B.,Moreau, J.F.,Stuart, D.I.,Heath, J.K.,Jones, E.Y.
The crystal structure and biological function of leukemia inhibitory factor: implications for receptor binding.
Cell(Cambridge,Mass.), 77:1101-1116, 1994

PubMed Abstract: The structure of murine leukemia inhibitory factor (LIF) has been determined by X-ray crystallography at 2.0 A resolution. The main chain fold conforms to the four alpha-helix bundle topology previously observed for several members of the hematopoietic cytokine family. Of these, LIF shows closest structural homology to granulocyte colony-stimulating factor and growth hormone (GH). Sequence alignments for the functionally related molecules oncostatin M and ciliary neurotrophic factor, when mapped to the LIF structure, indicate regions of conserved surface character. Analysis of the biological function and receptor specificity of a series of human-mouse LIF chimeras implicate two regions of receptor interaction that are located in the fourth helix and the preceding loop. A model for receptor binding based on the structure of the GH ligand-receptor complex requires additional, novel features to account for these data.

PubMed: 8020098
DOI: 10.1016/0092-8674(94)90449-9

実験手法

X-RAY DIFFRACTION (2 Å)