1LJM

DNA recognition is mediated by conformational transition and by DNA bending

Summary for 1LJM

• Entry DOI: 10.2210/pdb1ljm/pdb
• Descriptor: RUNX1 transcription factor, CHLORIDE ION (3 entities in total)
• Functional Keywords: immunoglobulin fold, beta-sandwich, transcription
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: Q01196
• Total number of polymer chains: 2
• Total formula weight: 29336.61

Authors

Bartfeld, D.,Shimon, L.,Couture, G.C.,Rabinovich, D.,Frolow, F.,Levanon, D.,Groner, Y.,Shakked, Z. (deposition date: 2002-04-22, release date: 2002-11-06, Last modification date: 2023-08-16)

Primary citation

Bartfeld, D.,Shimon, L.,Couture, G.,Rabinovich, D.,Frolow, F.,Levanon, D.,Groner, Y.,Shakked, Z.
DNA Recognition by the RUNX1 Transcription Factor Is Mediated by an Allosteric Transition in the RUNT Domain and by DNA Bending.
Structure, 10:1395-1407,

PubMed Abstract: The Runt domain proteins are transcription regulators of major developmental pathways. Here we present the crystal structures of the Runt domain (RD) of the human protein RUNX1 and its DNA binding site in their free states and compare them with the published crystal structures of RD bound to DNA and to the partner protein CBFbeta. We demonstrate that (1) RD undergoes an allosteric transition upon DNA binding, which is further stabilized by CBFbeta, and that (2) the free DNA target adopts a bent-helical conformation compatible with that of the complex. These findings elucidate the mechanism by which CBFbeta enhances RD binding to DNA as well as the role of the intrinsic conformation of the DNA target in the recognition process.

PubMed: 12377125
DOI: 10.1016/S0969-2126(02)00853-5

Experimental method

X-RAY DIFFRACTION (2.5 Å)