1LEI

The kB DNA sequence from the HLV-LTR functions as an allosteric regulator of HIV transcription

Summary for 1LEI

• Entry DOI: 10.2210/pdb1lei/pdb
• Related: 1VKX
• Descriptor: 5'-D(*CP*TP*CP*AP*GP*GP*GP*AP*AP*AP*GP*TP*AP*CP*AP*GP*A)-3', 5'-D(*TP*CP*TP*GP*5ITP*AP*CP*5ITP*5ITP*5ITP*CP*CP*CP*TP*GP*AP*G)-3', NUCLEAR FACTOR NF-KAPPA-B P65 SUBUNIT, ... (5 entities in total)
• Functional Keywords: transcription factor, nf-kb-dna complex, transcription-dna complex, transcription/dna
• Biological source: Mus musculus (house mouse); More
• Cellular location: Nucleus: 1LEI; Nucleus. Isoform 5: Cytoplasm. Isoform 6: Nucleus. Isoform 7: Nucleus: 1LEI
• Total number of polymer chains: 4
• Total formula weight: 77032.56

Authors

Chen-Park, F.,Huang, D.B.,Ghosh, G. (deposition date: 2002-04-09, release date: 2003-04-15, Last modification date: 2024-11-20)

Primary citation

Chen-Park, F.E.,Huang, D.B.,Noro, B.,Thanos, D.,Ghosh, G.
The kB DNA sequence from the HIV Long Terminal Repeat functions as an allosteric regulator of HIV transcription
J.Biol.Chem., 277:24701-24708, 2002

PubMed Abstract: NF-kappaB is an inducible transcription factor involved in the immune response, inflammation, and viral transcription. To address how the two NF-kappaB and three Sp1 binding sites of the human immunodeficiency virus (HIV) long terminal repeat (LTR) control multiple activator assembly and transcription, we first observed and compared unique conformations between the crystallographic structure of the NF-kappaB p50.p65 heterodimer bound to the uPA-kappaB target site to that of the p50.p65.HIV-kappaB complex. Next, cooperativity between two NF-kappaB molecules bound to tandem HIV-kappaB sequences was measured as well as that of NF-kappaB and transcription factor Sp1 when bound to adjacent sites. The cooperativity of hybrid HIV-LTR enhancers was measured with the 3' kappaB site converted to uPA-kappaB or to interferon beta gene enhancer kappaB. The hybrids were defective in transcriptional activator assembly and less active transcriptionally. These functional differences correlate with observed conformational differences and demonstrate that distinct kappaB DNA sequences function as allosteric regulators in a gene-specific manner.

PubMed: 11970949
DOI: 10.1074/jbc.M200007200

Experimental method

X-RAY DIFFRACTION (2.7 Å)