1L9U

THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION

1L9U の概要

• エントリーDOI: 10.2210/pdb1l9u/pdb
• 分子名称: RNA POLYMERASE, ALPHA SUBUNIT, RNA POLYMERASE, BETA SUBUNIT, RNA POLYMERASE, BETA-PRIME SUBUNIT, ... (7 entities in total)
• 機能のキーワード: helix-turn-helix, coiled-coil, transcription
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 831268.95

構造登録者

Murakami, K.S.,Masuda, S.,Darst, S.A. (登録日: 2002-03-26, 公開日: 2002-05-22, 最終更新日: 2024-02-14)

主引用文献

Murakami, K.S.,Masuda, S.,Darst, S.A.
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution.
Science, 296:1280-1284, 2002

PubMed Abstract: The crystal structure of the initiating form of Thermus aquaticus RNA polymerase, containing core RNA polymerase (alpha2betabeta'omega) and the promoter specificity sigma subunit, has been determined at 4 angstrom resolution. Important structural features of the RNA polymerase and their roles in positioning sigma within the initiation complex are delineated, as well as the role played by sigma in modulating the opening of the RNA polymerase active-site channel. The two carboxyl-terminal domains of sigma are separated by 45 angstroms on the surface of the RNA polymerase, but are linked by an extended loop. The loop winds near the RNA polymerase active site, where it may play a role in initiating nucleotide substrate binding, and out through the RNA exit channel. The advancing RNA transcript must displace the loop, leading to abortive initiation and ultimately to sigma release.

PubMed: 12016306
DOI: 10.1126/science.1069594

実験手法

X-RAY DIFFRACTION (4 Å)