1L8C

STRUCTURAL BASIS FOR HIF-1ALPHA/CBP RECOGNITION IN THE CELLULAR HYPOXIC RESPONSE

Summary for 1L8C

• Entry DOI: 10.2210/pdb1l8c/pdb
• NMR Information: BMRB: 5327
• Descriptor: CREB-binding protein, Hypoxia-inducible factor 1 alpha, ZINC ION (3 entities in total)
• Functional Keywords: gene regulation
• Biological source: Mus musculus (house mouse); More
• Cellular location: Cytoplasm (By similarity): P45481; Cytoplasm: Q16665
• Total number of polymer chains: 2
• Total formula weight: 16615.12

Authors

Dames, S.A.,Martinez-Yamout, M.,De Guzman, R.N.,Dyson, H.J.,Wright, P.E. (deposition date: 2002-03-19, release date: 2002-04-10, Last modification date: 2024-05-22)

Primary citation

Dames, S.A.,Martinez-Yamout, M.,De Guzman, R.N.,Dyson, H.J.,Wright, P.E.
Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response.
Proc.Natl.Acad.Sci.USA, 99:5271-5276, 2002

PubMed Abstract: The cellular response to low tissue oxygen concentrations is mediated by the hypoxia-inducible transcription factor HIF-1. Under hypoxic conditions, HIF-1 activates transcription of critical adaptive genes by recruitment of the general coactivators CBP/p300 through interactions with its alpha-subunit (Hif-1 alpha). Disruption of the Hif-1 alpha/p300 interaction has been linked to attenuation of tumor growth. To delineate the structural basis for this interaction, we have determined the solution structure of the complex between the carboxy-terminal activation domain (CAD) of Hif-1 alpha and the zinc-binding TAZ1 (CH1) motif of cyclic-AMP response element binding protein (CREB) binding protein (CBP). Despite the overall similarity of the TAZ1 structure to that of the TAZ2 (part of the CH3) domain of CBP, differences occur in the packing of helices that can account for differences in specificity. The unbound CAD is intrinsically disordered and remains relatively extended upon binding, wrapping almost entirely around the TAZ1 domain in a groove through much of its surface. Three short helices are formed upon binding, stabilized by intermolecular interactions. The Asn-803 side chain, which functions as a hypoxic switch, is located on the second of these helices and is buried in the molecular interface. The third helix of the Hif-1 alpha CAD docks in a deep hydrophobic groove in TAZ1, providing extensive intermolecular hydrophobic interactions that contribute to the stability of the complex. The structure of this complex provides new insights into the mechanism through which Hif-1 alpha recruits CBP/p300 in response to hypoxia.

PubMed: 11959977
DOI: 10.1073/pnas.082121399

Experimental method

SOLUTION NMR