1KR5

Crystal structure of human L-isoaspartyl methyltransferase

Summary for 1KR5

• Entry DOI: 10.2210/pdb1kr5/pdb
• Descriptor: Protein-L-isoaspartate O-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE (3 entities in total)
• Functional Keywords: rossmann-fold doubly-wound-alpha-beta-alpha-sandwich, transferase
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P22061
• Total number of polymer chains: 1
• Total formula weight: 24921.62

Authors

Ryttersgaard, C.,Griffith, S.C.,Sawaya, M.R.,MacLaren, D.C.,Clarke, S.,Yeates, T.O. (deposition date: 2002-01-08, release date: 2002-02-08, Last modification date: 2023-09-20)

Primary citation

Ryttersgaard, C.,Griffith, S.C.,Sawaya, M.R.,MacLaren, D.C.,Clarke, S.,Yeates, T.O.
Crystal structure of human L-isoaspartyl methyltransferase.
J.Biol.Chem., 277:10642-10646, 2002

PubMed Abstract: The enzyme l-isoaspartyl methyltransferase initiates the repair of damaged proteins by recognizing and methylating isomerized and racemized aspartyl residues in aging proteins. The crystal structure of the human enzyme containing a bound S-adenosyl-l-homocysteine cofactor is reported here at a resolution of 2.1 A. A comparison of the human enzyme to homologs from two other species reveals several significant differences among otherwise similar structures. In all three structures, we find that three conserved charged residues are buried in the protein interior near the active site. Electrostatics calculations suggest that these buried charges might make significant contributions to the energetics of binding the charged S-adenosyl-l-methionine cofactor and to catalysis. We suggest a possible structural explanation for the observed differences in reactivity toward the structurally similar l-isoaspartyl and d-aspartyl residues in the human, archael, and eubacterial enzymes. Finally, the human structure reveals that the known genetic polymorphism at residue 119 (Val/Ile) maps to an exposed region away from the active site.

PubMed: 11792715
DOI: 10.1074/jbc.M200229200

Experimental method

X-RAY DIFFRACTION (2.1 Å)