1KK1

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant complexed with GDPNP-Mg2+

Summary for 1KK1

• Entry DOI: 10.2210/pdb1kk1/pdb
• Related: 1KJZ 1KKO
• Descriptor: eIF2gamma, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: initiation of translation, translation
• Biological source: Pyrococcus abyssi
• Total number of polymer chains: 1
• Total formula weight: 45531.91

Authors

Schmitt, E.,Blanquet, S.,Mechulam, Y. (deposition date: 2001-12-06, release date: 2002-04-10, Last modification date: 2023-08-16)

Primary citation

Schmitt, E.,Blanquet, S.,Mechulam, Y.
The large subunit of initiation factor aIF2 is a close structural homologue of elongation factors.
EMBO J., 21:1821-1832, 2002

PubMed Abstract: The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged.

PubMed: 11927566
DOI: 10.1093/emboj/21.7.1821

Experimental method

X-RAY DIFFRACTION (1.8 Å)