1KHW
Crystal Structure of Rabbit Hemorrhagic Disease Virus RNA-dependent RNA polymerase complexed with Mn2+
Summary for 1KHW
| Entry DOI | 10.2210/pdb1khw/pdb |
| Related | 1KHV |
| Descriptor | RNA-DIRECTED RNA POLYMERASE, MANGANESE (II) ION (3 entities in total) |
| Functional Keywords | rna-dependent rna polymerase, transferase |
| Biological source | Rabbit hemorrhagic disease virus |
| Total number of polymer chains | 2 |
| Total formula weight | 115882.50 |
| Authors | Ng, K.K.,Cherney, M.M.,Vazquez, A.L.,Machin, A.,Alonso, J.M.,Parra, F.,James, M.N. (deposition date: 2001-12-01, release date: 2002-01-16, Last modification date: 2024-11-06) |
| Primary citation | Ng, K.K.,Cherney, M.M.,Vazquez, A.L.,Machin, A.,Alonso, J.M.,Parra, F.,James, M.N. Crystal structures of active and inactive conformations of a caliciviral RNA-dependent RNA polymerase. J.Biol.Chem., 277:1381-1387, 2002 Cited by PubMed Abstract: The structure of the RNA-dependent RNA polymerase (RdRP) from the rabbit hemorrhagic disease virus has been determined by x-ray crystallography to a 2.5-A resolution. The overall structure resembles a "right hand," as seen before in other polymerases, including the RdRPs of polio virus and hepatitis C virus. Two copies of the polymerase are present in the asymmetric unit of the crystal, revealing active and inactive conformations within the same crystal form. The fingers and palm domains form a relatively rigid unit, but the thumb domain can adopt either "closed" or "open" conformations differing by a rigid body rotation of approximately 8 degrees. Metal ions bind at different positions in the two conformations and suggest how structural changes may be important to enzymatic function in RdRPs. Comparisons between the structures of the alternate conformational states of rabbit hemorrhagic disease virus RdRP and the structures of RdRPs from hepatitis C virus and polio virus suggest novel structure-function relationships in this medically important class of enzymes. PubMed: 11677245DOI: 10.1074/jbc.M109261200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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