1KHW

Crystal Structure of Rabbit Hemorrhagic Disease Virus RNA-dependent RNA polymerase complexed with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2001-06-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	70.694, 119.845, 159.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.200 * - 2.700
R-factor	0.235 *
Rwork	0.238
R-free	0.27000 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.250 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.097	0.722
Total number of observations	219584 *
Number of reflections	37963
<I/σ(I)>	17.4	2.4
Completeness [%]	99.9	100
Redundancy	5.8	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 8000, sodium HEPES, manganese chloride glycerol, hexanediol, 3'-deoxy-ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	10-12 (%)
3	1	reservoir	Tris-Cl	0.1 (M)	pH7.5
4	1	reservoir	sodium thiocyanate	0.2 (M)
5	1	reservoir	L-proline	0.1 (M)
6	1	reservoir	glycerol	15 (%(w/v))
7	1	reservoir	1,6-hexanediol	7 (%(v/v))
8	1	reservoir	CHAPS	0.1 (%(w/v))
9	1	reservoir		5 (mM)