1KFO

CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION

Summary for 1KFO

• Entry DOI: 10.2210/pdb1kfo/pdb
• Descriptor: 5'-R(*GP*AP*AP*UP*GP*CP*CP*UP*GP*CP*GP*AP*GP*CP*AP*(5BU)P*CP*CP*C)-3' (2 entities in total)
• Functional Keywords: rna
• Total number of polymer chains: 1
• Total formula weight: 6131.56

Authors

Lima, S.,Hildenbrand, J.,Korostelev, A.,Hattman, S.,Li, H. (deposition date: 2001-11-21, release date: 2001-12-07, Last modification date: 2024-02-07)

Primary citation

Lima, S.,Hildenbrand, J.,Korostelev, A.,Hattman, S.,Li, H.
Crystal structure of an RNA helix recognized by a zinc-finger protein: an 18-bp duplex at 1.6 A resolution.
RNA, 8:924-932, 2002

PubMed Abstract: The crystal structure of the 19-mer RNA, 5'-GAAUGCCUGCGAGCAUCCC-3' has been determined from X-ray diffraction data to 1.6 A resolution by the multiwavelength anomalous diffraction method from crystals containing a brominated uridine. In the crystal, this RNA forms an 18-mer self-complementary double helix with the 19th nucleotide flipped out of the helix. This helix contains most of the target stem recognized by the bacteriophage Mu Com protein (control of mom), which activates translation of an unusual DNA modification enzyme, Mom. The 19-mer duplex, which contains one A.C mismatch and one A.C/G.U tandem wobble pair, was shown to bind to the Com protein by native gel electrophoresis shift assay. Comparison of the geometries and base stacking properties between Watson-Crick base pairs and the mismatches in the crystal structure suggest that both hydrogen bonding and base stacking are important for stabilizing these mismatched base pairs, and that the unusual geometry adopted by the A.C mismatch may reveal a unique structural motif required for the function of Com.

PubMed: 12166647
DOI: 10.1017/S1355838202028893

Experimental method

X-RAY DIFFRACTION (1.6 Å)