1KFO
CRYSTAL STRUCTURE OF AN RNA HELIX RECOGNIZED BY A ZINC-FINGER PROTEIN: AN 18 BASE PAIR DUPLEX AT 1.6 RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 45.336, 45.336, 95.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 1.600 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.28800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.211 * |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 * |
| High resolution limit [Å] | 1.600 * |
| Rmerge | 0.056 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5 (mM) | |
| 2 | 1 | drop | cacodylate acid | 50 (mM) | pH6.5 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 2.5-5 (mM) | or CrSO4 | |
| 5 | 1 | drop | 0.8-1.1 (M) | ||
| 6 | 1 | reservoir | cacodylate acid | 100 (mM) | pH6.5 |
| 7 | 1 | reservoir | 5 (mM) | ||
| 8 | 1 | reservoir | 1.9-2.2 (mM) |
