1K83

Crystal Structure of Yeast RNA Polymerase II Complexed with the Inhibitor Alpha Amanitin

Summary for 1K83

• Entry DOI: 10.2210/pdb1k83/pdb
• Related: 1I50 2VUM 3CQZ
• Related PRD ID: PRD_000201
• Descriptor: DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT, DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE, ALPHA AMANITIN, ... (14 entities in total)
• Functional Keywords: transcription-toxin complex, alpha amanitin, toxin, inhibitor, polymerase, transferase, dna binding, zinc-finger, phosphoprotein, transcription, ubl transcription-toxin complex, transcription/toxin
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Nucleus: P04050 P08518 P16370 P20434 P20436 P27999 P38902; Nucleus, nucleolus: P40422 P22139; Cytoplasm: P20435
• Total number of polymer chains: 11
• Total formula weight: 471143.19

Authors

Bushnell, D.A.,Cramer, P.,Kornberg, R.D. (deposition date: 2001-10-22, release date: 2002-02-13, Last modification date: 2023-08-16)

Primary citation

Bushnell, D.A.,Cramer, P.,Kornberg, R.D.
Structural Basis of Transcription: Alpha-Amanitin-RNA Polymerase II Cocrystal at 2.8 A Resolution.
Proc.Natl.Acad.Sci.USA, 99:1218-, 2002

PubMed Abstract: The structure of RNA polymerase II in a complex with the inhibitor alpha-amanitin has been determined by x-ray crystallography. The structure of the complex indicates the likely basis of inhibition and gives unexpected insight into the transcription mechanism.

PubMed: 11805306
DOI: 10.1073/PNAS.251664698

Experimental method

X-RAY DIFFRACTION (2.8 Å)