1K83
Crystal Structure of Yeast RNA Polymerase II Complexed with the Inhibitor Alpha Amanitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 122.510, 222.480, 374.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i50 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.067 | 0.216 |
| Number of reflections | 124441 | 12292 * |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | Fu, J., (1999) Cell, 98, 799. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg) | |
| 2 | 1 | reservoir | dioxane | 50 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | 390 (mM) | pH6.5 | |
| 5 | 1 | reservoir | PEG6000 |
