1K3Z

X-ray crystal structure of the IkBb/NF-kB p65 homodimer complex

1K3Z の概要

• エントリーDOI: 10.2210/pdb1k3z/pdb
• 分子名称: Transcription factor p65, transcription factor inhibitor I-kappa-B-beta (3 entities in total)
• 機能のキーワード: protein-protein complex, transcription factors, nuclear localization, transcription
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Nucleus: Q04207; Cytoplasm: Q60778
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62025.21

構造登録者

Shiva, M.,Huang, D.B.,Chen, Y.,Huxford, T.,Ghosh, S.,Ghosh, G. (登録日: 2001-10-04, 公開日: 2002-10-04, 最終更新日: 2023-08-16)

主引用文献

Malek, S.,Huang, D.B.,Huxford, T.,Ghosh, S.,Ghosh, G.
X-ray crystal structure of an IkappaBbeta x NF-kappaB p65 homodimer complex.
J.Biol.Chem., 278:23094-23100, 2003

PubMed Abstract: We report the crystal structure of a murine IkappaBbeta x NF-kappaB p65 homodimer complex. Crystallographic models were determined for two triclinic crystalline systems and refined against data at 2.5 and 2.1 A. The overall complex structure is similar to that of the IkappaBalpha.NF-kappaB p50/p65 heterodimer complex. One NF-kappaB p65 subunit nuclear localization signal clearly contacts IkappaBbeta, whereas a homologous segment from the second subunit of the homodimer is mostly solvent-exposed. The unique 47-amino acid insertion between ankyrin repeats three and four of IkappaBbeta is mostly disordered in the structure. Primary sequence analysis and differences in the mode of binding at the IkappaBbeta sixth ankyrin repeat and NF-kappaB p65 homodimer suggest a model for nuclear IkappaBbeta.NF-kappaB.DNA ternary complex formation. These unique structural features of IkappaBbeta may contribute to its ability to mediate persistent NF-kappaB activation.

PubMed: 12686541
DOI: 10.1074/jbc.M301022200

実験手法

X-RAY DIFFRACTION (2.5 Å)