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1K3Z

X-ray crystal structure of the IkBb/NF-kB p65 homodimer complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]110
Detector technologyAREA DETECTOR
Collection date2000-04-15
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameP 1
Unit cell lengths46.400, 48.920, 59.470
Unit cell angles95.17, 91.80, 105.45
Refinement procedure
Resolution30.000 - 2.500
Rwork0.181
R-free0.27900

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)IkBalpha/p65dd (pdb entry 1ikn)
RMSD bond length0.007
RMSD bond angle1.340

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.600
High resolution limit [Å]2.5002.500
Rmerge0.0790.253
Total number of observations110726

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Number of reflections16126

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<I/σ(I)>7.93
Completeness [%]93.0

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72
Redundancy7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

5.6

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG80008-10 (%)
21reservoirsodium citratepH5.6

227111

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