1K3Z
X-ray crystal structure of the IkBb/NF-kB p65 homodimer complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | AREA DETECTOR |
Collection date | 2000-04-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 46.400, 48.920, 59.470 |
Unit cell angles | 95.17, 91.80, 105.45 |
Refinement procedure
Resolution | 30.000 - 2.500 |
Rwork | 0.181 |
R-free | 0.27900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IkBalpha/p65dd (pdb entry 1ikn) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.079 | 0.253 |
Total number of observations | 110726 * | |
Number of reflections | 16126 * | |
<I/σ(I)> | 7.9 | 3 |
Completeness [%] | 93.0 * | 72 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 8-10 (%) | |
2 | 1 | reservoir | sodium citrate | pH5.6 |