1K3B

Crystal Structure of Human Dipeptidyl Peptidase I (Cathepsin C): Exclusion Domain Added to an Endopeptidase Framework Creates the Machine for Activation of Granular Serine Proteases

1K3B の概要

• エントリーDOI: 10.2210/pdb1k3b/pdb
• 分子名称: dipeptydil-peptidase I exclusion domain, dipeptydil-peptidase I light chain, dipeptydil-peptidase I heavy chain, ... (7 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 40120.33

構造登録者

Turk, D.,Janjic, V.,Stern, I.,Podobnik, M.,Lamba, D.,Dahl, S.W.,Lauritzen, C.,Pedersen, J.,Turk, V.,Turk, B. (登録日: 2001-10-02, 公開日: 2002-04-02, 最終更新日: 2024-10-30)

主引用文献

Turk, D.,Janjic, V.,Stern, I.,Podobnik, M.,Lamba, D.,Dahl, S.W.,Lauritzen, C.,Pedersen, J.,Turk, V.,Turk, B.
Structure of human dipeptidyl peptidase I (cathepsin C): exclusion domain added to an endopeptidase framework creates the machine for activation of granular serine proteases.
EMBO J., 20:6570-6582, 2001

PubMed Abstract: Dipeptidyl peptidase I (DPPI) or cathepsin C is the physiological activator of groups of serine proteases from immune and inflammatory cells vital for defense of an organism. The structure presented shows how an additional domain transforms the framework of a papain-like endopeptidase into a robust oligomeric protease-processing enzyme. The tetrahedral arrangement of the active sites exposed to solvent allows approach of proteins in their native state; the massive body of the exclusion domain fastened within the tetrahedral framework excludes approach of a polypeptide chain apart from its termini; and the carboxylic group of Asp1 positions the N-terminal amino group of the substrate. Based on a structural comparison and interactions within the active site cleft, it is suggested that the exclusion domain originates from a metallo-protease inhibitor. The location of missense mutations, characterized in people suffering from Haim-Munk and Papillon-Lefevre syndromes, suggests how they disrupt the fold and function of the enzyme.

PubMed: 11726493
DOI: 10.1093/emboj/20.23.6570

実験手法

X-RAY DIFFRACTION (2.15 Å)