1K1Q

Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus

Summary for 1K1Q

• Entry DOI: 10.2210/pdb1k1q/pdb
• Related: 1K1S
• Descriptor: DBH protein (2 entities in total)
• Functional Keywords: dna polymerase, error-prone polymerase, lesion-bypass polymerase, transcription
• Biological source: Sulfolobus solfataricus
• Cellular location: Cytoplasm (Probable): P96022
• Total number of polymer chains: 2
• Total formula weight: 80059.55

Authors

Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T. (deposition date: 2001-09-25, release date: 2001-10-31, Last modification date: 2024-02-07)

Primary citation

Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T.
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus.
Nat.Struct.Biol., 8:984-989, 2001

PubMed Abstract: A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.

PubMed: 11685247
DOI: 10.1038/nsb1101-984

Experimental method

X-RAY DIFFRACTION (2.8 Å)