1K1Q
Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-23 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 111.715, 111.715, 132.175 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.100 - 2.800 |
| R-factor | 0.26 * |
| Rwork | 0.263 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 | 0.317 |
| Number of reflections | 21610 | |
| <I/σ(I)> | 16.6 | 2.1 |
| Completeness [%] | 94.1 | 71.3 |
| Redundancy | 3.5 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | PEG2000, sodium fluoride, magnesium chloride, cacodylate, glycerol, sucrose monolaurate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG2000 | 20 (%(w/v)) | |
| 10 | 1 | reservoir | glycerol | 40 (%(v/v)) | |
| 2 | 1 | drop | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | sucrose monolaurate | 0.2 (mM) | |
| 6 | 1 | reservoir | PEG2000 | 10 (%(v/v)) | |
| 7 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 8 | 1 | reservoir | 0.1 (M) | ||
| 9 | 1 | reservoir | 5 (mM) |
