1JUM

Crystal structure of the multidrug binding transcriptional repressor QacR bound to the natural drug berberine

Summary for 1JUM

• Entry DOI: 10.2210/pdb1jum/pdb
• Related: 1JT0 1JT6 1JTX 1JTY 1JUP 1JUS
• Descriptor: HYPOTHETICAL TRANSCRIPTIONAL REGULATOR IN QACA 5'REGION, SULFATE ION, BERBERINE, ... (4 entities in total)
• Functional Keywords: multidrug binding, berberine, plant alkaloid, natural drug, qacr, multidrug recognition, s. aureus, transcription
• Biological source: Staphylococcus aureus
• Total number of polymer chains: 4
• Total formula weight: 94381.84

Authors

Schumacher, M.A.,Miller, M.C.,Grkovic, S.,Brown, M.H.,Skurray, R.A.,Brennan, R.G. (deposition date: 2001-08-24, release date: 2001-12-12, Last modification date: 2023-08-16)

Primary citation

Schumacher, M.A.,Miller, M.C.,Grkovic, S.,Brown, M.H.,Skurray, R.A.,Brennan, R.G.
Structural mechanisms of QacR induction and multidrug recognition.
Science, 294:2158-2163, 2001

PubMed Abstract: The Staphylococcus aureus multidrug binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by structurally diverse cationic lipophilic drugs. Here, we report the crystal structures of six QacR-drug complexes. Compared to the DNA bound structure, drug binding elicits a coil-to-helix transition that causes induction and creates an expansive multidrug-binding pocket, containing four glutamates and multiple aromatic and polar residues. These structures indicate the presence of separate but linked drug-binding sites within a single protein. This multisite drug-binding mechanism is consonant with studies on multidrug resistance transporters.

PubMed: 11739955
DOI: 10.1126/science.1066020

Experimental method

X-RAY DIFFRACTION (2.98 Å)