1JT6

Crystal structure of the multidrug binding protein QacR bound to dequalinium

1JT6 の概要

• エントリーDOI: 10.2210/pdb1jt6/pdb
• 関連するPDBエントリー: 1JT0 1JTY 1JUM 1JUP 1JUS
• 分子名称: Hypothetical transcriptional regulator IN QACA 5'region, SULFATE ION, DEQUALINIUM, ... (4 entities in total)
• 機能のキーワード: mutlidrug binding recognition, qacr, mulitdrug resistance, s. aureus, transcription
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 91763.00

構造登録者

Schumacher, M.A.,Miller, M.C.,Grkovic, S.,Brown, M.H.,Skurray, R.A.,Brennan, R.G. (登録日: 2001-08-20, 公開日: 2001-12-12, 最終更新日: 2024-04-03)

主引用文献

Schumacher, M.A.,Miller, M.C.,Grkovic, S.,Brown, M.H.,Skurray, R.A.,Brennan, R.G.
Structural mechanisms of QacR induction and multidrug recognition.
Science, 294:2158-2163, 2001

PubMed Abstract: The Staphylococcus aureus multidrug binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by structurally diverse cationic lipophilic drugs. Here, we report the crystal structures of six QacR-drug complexes. Compared to the DNA bound structure, drug binding elicits a coil-to-helix transition that causes induction and creates an expansive multidrug-binding pocket, containing four glutamates and multiple aromatic and polar residues. These structures indicate the presence of separate but linked drug-binding sites within a single protein. This multisite drug-binding mechanism is consonant with studies on multidrug resistance transporters.

PubMed: 11739955
DOI: 10.1126/science.1066020

実験手法

X-RAY DIFFRACTION (2.54 Å)