1JGG

Even-skipped Homeodomain Complexed to AT-rich DNA

Summary for 1JGG

• Entry DOI: 10.2210/pdb1jgg/pdb
• Descriptor: 5'-D(P*TP*AP*AP*TP*TP*GP*AP*AP*TP*T)-3', 5'-D(P*AP*AP*TP*TP*CP*AP*AP*TP*TP*A)-3', Segmentation Protein Even-Skipped, ... (4 entities in total)
• Functional Keywords: even-skipped, homeodomain, protein-dna complex, transcription-dna complex, transcription/dna
• Biological source: Drosophila melanogaster (fruit fly)
• Cellular location: Nucleus: P06602
• Total number of polymer chains: 4
• Total formula weight: 21186.51

Authors

Hirsch, J.A.,Aggarwal, A.K. (deposition date: 2001-06-25, release date: 2001-07-06, Last modification date: 2024-02-07)

Primary citation

Hirsch, J.A.,Aggarwal, A.K.
Structure of the even-skipped homeodomain complexed to AT-rich DNA: new perspectives on homeodomain specificity.
EMBO J., 14:6280-6291, 1995

PubMed Abstract: even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 A resolution. The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity. In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases.

PubMed: 8557047

Experimental method

X-RAY DIFFRACTION (2 Å)