1JGG
Even-skipped Homeodomain Complexed to AT-rich DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Detector | FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.060, 61.610, 39.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.229 * |
Rwork | 0.229 |
R-free | 0.31600 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.560 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.076 | 0.186 |
Total number of observations | 20689 * | |
Number of reflections | 8613 | |
Completeness [%] | 77.1 | 52.4 |
Redundancy | 2.4 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.4 | 20 * | Hirsch, J.A., (1995) Proteins, 21, 268. * |
1 | Vapor diffusion, sitting drop * | 6.4 | 20 * | Hirsch, J.A., (1995) Proteins, 21, 268. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium acetate | ||
2 | 1 | 1 | potassium chloride | ||
3 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 7-15 (%) | ||
2 | 1 | reservoir | acetate acid | 4 (M) | |
3 | 1 | reservoir | dithiothreitol | 3 (mM) |