1JGG
Even-skipped Homeodomain Complexed to AT-rich DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.060, 61.610, 39.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.31600 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.560 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.186 |
| Total number of observations | 20689 * | |
| Number of reflections | 8613 | |
| Completeness [%] | 77.1 | 52.4 |
| Redundancy | 2.4 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.4 | 20 * | Hirsch, J.A., (1995) Proteins, 21, 268. * |
| 1 | Vapor diffusion, sitting drop * | 6.4 | 20 * | Hirsch, J.A., (1995) Proteins, 21, 268. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | potassium chloride | ||
| 3 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 7-15 (%) | ||
| 2 | 1 | reservoir | acetate acid | 4 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 3 (mM) |
